【摘要】 目的 :建立适宜的小鼠胚胎体外培养体系 ,探讨CO2 气体纯度和培养器皿对小鼠胚胎体外发育的影响。 方法 :采用微滴法培养 2 细胞鼠胚 ,以 4～ 8 细胞鼠胚和桑椹胚形成率作为判断培养效果的指标。 结果 :普通纯度CO2 气体培养组的 4～ 8 细胞形成率、桑椹胚形成率分别为 12 .5 %、0 .0 % ,高纯度CO2 气体培养组分别为6 7.6 %、4 4 .1% ,两组比较差异有统计学意义 (P均 <0 .0 0 1)。国产玻璃器皿培养组的 4～ 8 细胞形成率、桑椹胚形成率分别为 74 .4 %、5 3.5 % ,进口一次性器皿培养组分别为 85 .7%、6 2 .8% ,两组比较差异无统计学意义 (P >0 .0 5 )。结论 :高纯度CO2 气体更适宜鼠胚的体外发育 ;国产玻璃器皿与进口一次性器皿对鼠胚体外发育的效果相近更多还原

【Abstract】 Objective: To establish more suitable in vitro culture system of embryos, studied the effects of purity of CO\-2gas and different types of culture dishes on in vitro development of mouse embryos. Methods: 2 cell mouse embryos were cultured in microdrops of medium and the percentage of embryos developed to 4～8 cell embryos and morular stage was used as evaluated criteria. Results: (1) There were significantly higher 4～8 cell embryos, morula percentages in high purity CO\-2gas group (67.6%, 44.1% respectively) as compared those in common purity CO\-2gas group (12.5%, 0.0% respectively). (2) There were no significant difference 4～8 cell embryos, morula percentages between the homemade culture dishes group (74.4%, 53.5% respectively) and the imported disposal culture dishes group (85.7%, 62.8% respectively). Conclusions: High purity CO\-2gas was more suitable in supporting in vitro development for mouse embryos. Both the homemade and the imported disposal culture dishes produced good results of embryos in vitro development.更多还原