【摘要】 用超声波脂质体制备法建立一种阿霉素脂质体的制备方法,并观察其体外对人肝癌细胞系n9101及人低分化胃癌MGC803细胞系的杀伤效力.对阿霉素脂质体溶液进行扫描,寻求最佳波长.用sephadexG50柱分离单纯阿霉素和阿霉素脂质体.苔盼蓝拒染法测定杀伤力.阿霉素脂质体在480nm处有最大吸收峰,测定阿霉素脂质体的包封率为99.39%.对n9101和MGC803两种细胞系的杀伤作用与单纯阿霉素相近,作用更持久.此法制备的脂质体包封率高,重现性好,简便易行,所制得的阿霉素脂质体具有高度的杀伤活性.更多还原

【Abstract】 A method for the preparation of adriamycin liposomes ( ADML ) by ultrosonic agitation was established, followed by the study of the cytotoxocity of ADML on n 9101 and MGC 803 cell lines in vitro. The adriamycin solutions were investigated by spectrophotometric method and the maximum absorption peaks were founded. Adriamycinliposomes was purified by exclusion chromatography using Sephadex G50. Cytotoxicity assay was performed by the tryphan exclusion test. The spectrum indicated that the maximum absorption of adiamycin was at 480 nm. The encapulation efficiency of adriamycin was 99.39%. Cytotoxicity on n9101 and MGC 803 of adiamycinliposome was found to be similar to that of free adriamycin, but the effect lasted more longer. The method for the preparation of ADML in this work is easy to be performed and bears the merits of high encapulation efficiency, reproducibility and cytotoxicity.更多还原