【摘要】 目的　观察七十味珍珠丸的抗惊厥作用及作用机制。方法　七十味珍珠丸ig给药 ,使用硫代氨基脲制成惊厥小鼠模型 ,测定惊厥开始时间、致死率 ;采用高压液相色谱仪测定小鼠脑内抑制性氨基酸γ 氨基丁酸 (GABA)、甘氨酸 (Gly)和兴奋性氨基酸谷氨酸 (Glu)、天门冬氨酸 (Asp)的含量。 结果　七十味珍珠丸对硫代氨基脲所致惊厥的开始时间 ,具有显著延迟作用 ,可明显减少小鼠致死率。有升高惊厥小鼠脑内GABA ,降低脑内Glu含量的作用趋势 ,但无显著差异 (P >0 .0 5 ) ,而对Glu/GABA的比值 ,却有明显的降低作用 (P <0 .0 5、P <0 .0 1) ,使Glu/GABA保持正常动物的水平 ,即维持中枢神经系统兴奋和抑制状态平衡的作用 ,阻止惊厥发生。结论　该药具有抗惊厥作用 ,其作用机制可能是通过调节脑内兴奋性和抑制性氨基酸的平衡来实现的更多还原

【Abstract】 OBJECTIVE To study the effects of Rannasangpei on level of amino acid in brain of mice with eclampsia. METHOD The level of GABA, Gly, Glu and Asp in brain of mice, who were the model with eclampsia thiocarbamate induced, were determined using high performance liquid chromatography (HPLC) while changes of attack time and fatal rate were examined.RESULTS The level of GABA was increased by administration of Rannasangpei and inversely in Glu, but no statistic difference ( P >0.05). The value of Glu/GABA was decreased significantly ( P <0.05 or 0.01). And the eclampsia time was delayed and the fatal rate decreased remarkably.CONCLUSION The Rannasangpai could decrease the value of Glu/GABA to maintain the ratio to a normal level and a balance between excitatory and inhibitory state in CNS of mice with eclampsia.更多还原