【摘要】 目的:通过对人遗传性骨软骨瘤软骨细胞的体外培养,观察肿瘤软骨细胞的生长特性及相关生物学特点,为进一步研究骨软骨瘤细胞学起因奠定基础。方法:原代培养遗传性骨软骨瘤组织中的软骨细胞并进行传代,进行活细胞形态观察,同时测定细胞增殖能力及贴壁率,与正常人关节软骨细胞做对照。结果:原代细胞在经过完全消化后,细胞活力仍达到95%。传代后骨软骨瘤细胞体积增大,细胞呈现星形,树突增多,细胞内颗粒多且明显。骨软骨瘤细胞增殖随着传代的增加减慢,第2代细胞增殖能力已经与原代细胞产生明显差别(P<0.01,t=3.203)。原代骨软骨瘤细胞在24h内大部分贴壁,随着传代的增加,细胞贴附能力下降,第4代细胞贴壁率与原代细胞产生明显区别(P<0.01,t=4.611)。结论:体外培养的骨软骨瘤细胞从形态,生物学特性(细胞增殖情况、细胞贴附能力)均不同于正常软骨细胞,为今后更加深入的研究遗传骨软骨瘤的病因学奠定一定的体外培养基础。更多还原

【Abstract】 AIM:To observe growth and related biological characters by culturing chondrocy tes of hereditary multiple exostosis in vitro from human and to establish bases for further research on the etiology of hereditary multiple exostosis. METHODS:Chondrocytes were culture primarily from exostosis and passaged. The m orphology of chondrocytes was observed by microscope. Meanwhile the abilities of cell proliferation and adhesive rates were detected and were compared with cont rol cartilage chondrocytes. RESULTS:The living chondrocytes still were 95%after complete digestion. After passaged, exostosis chondrocytes grew in mololayer culture had a stellate appea rance with more sticklike structures in the cytoplasm and had many obvious granu les. Proliferation of exostosis chondrocytes turned slowly with passaged increse d, and the obvious difference existed between the 2th passaged and the primary e xostosis chondrocytes(P< 0.01,t=3.203). Most of the primary exostosis chondrocyt es could adhere dishes within 24 hours. But with passaged incresed, the adhesive abilities turned down. The obvious difference existed between the 4th passaged and the primary exostosis chondrocytes.(P< 0.01,t=4.611).更多还原