【摘要】 目的观察中药合剂神经生长液对神经元生长的促进作用,为神经生长液用于治疗神经损伤、促进损伤神经修复提供实验依据。方法新生大鼠背根神经节无血清培养,在基础培养液中加入神经生长液,观察实验组与空白对照组背根神经节细胞生长状况和突起生长的长度。胎鼠大脑皮质神经元无血清培养,在基础培养液中加入神经生长液,采用3-(4,5-dimethylthiaz-2-y1)-2,5-dijohenyltetrazoliumbromide(MTT)法,观察实验组、神经生长因子(阳性对照)、空白对照组大脑皮质神经元的生长状态。结果与对照组相比,实验组背根神经节细胞突起生长明显,突起长度(314±43)μm显著大于对照组(76±26)μm(F=313.69,P<0.01);与对照组相比,神经生长因子、神经生长液均可显著提高无血清培养条件下大脑皮质神经元对MTT的代谢率(F=82.54,P均<0.01),且存在明显的剂量效应关系。结论神经生长液在体外可促进背根神经节和大脑皮质神经元生长。更多还原

【Abstract】 Aim To study the effects of Nerve Growth D ecotion(NGD),a traditional Chinese medicine mix ture,in promoting the growth of neuron as the basis of experimenta l evidences for NGD used to treat injury of nerve and promote recovery of injured nerve.Methods Neonatal rat dorsal root ganglia(DRG)cultured with free-serum plus NGD,w as used to observe the growth of DRG and the growth length of apophysis in ex-perimental group and control group w ith no NGD adding.The cerebral cortex neurons of the embryonic rat w ere cultured with free-serum plusNGD,using MTT observation method to observe the nerve growth factor(NGF)(positive control)of the experimental group and the gro wth of the cerebral cortex neurons of the contr ol group.Results The DRG growth of experimental group was remarkable a nd the growth length of apophysis was(314±43)μm,significantly higher than that of the control group(76±26)μm,F=313.69,P<0.01.Compared with the control group,both NGF and NGD effectively increased the metabolic rate of cerebral cortex neuron to MTT(F=82.54,P<0.01),with an evident rela-tionship with the doses.Conclusion NGD in vitro promotes the growth of the DRG and the cerebral cortex neuro ns.更多还原