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水稻耐冷性研究Ⅲ.特定颖花结实率作为耐冷性指标的分子依据

Studies on Cold Tolerance of Rice, Oryza sativa L. Ⅲ.Molecular Basis for Special Fertility Percentage as Evaluation Increterior of Cold Toleance

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【作者】 戴陆园林兴华叶昌荣加藤明齐藤浩二余腾琼徐福荣张端品

【Author】 DAI Lu-Yuan 1,2 LIN Xing-Hua 2 YE Chang-Rong 1 KATO Akira 3 SAITO Koji 3 YU Teng-Qiong 1 XU Fu-Rong 1 ZHANG Duan-Pin 2 ( 1 Crop Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205; 2 Huazhong Agricultural University, Wuhan, Hubei, 430070, China; 3 National Agriculture Research Center for the Hokkaido Region, Sapporo, Hokkaido, 062-8555, Japan)

【机构】 云南省农业科学院农作物品种资源站华中农业大学作物遗传改良国家重点实验室北海道国立农业研究中心华中农业大学作物遗传改良国家重点实验室 云南昆明650205湖北武汉430070云南昆明650205北海道0628555日本湖北武汉430070

【摘要】 以云南稻种耐冷性资源昆明小白谷及弱耐冷性的日本品种十和田配制的杂交F2 代为材料 ,采用 15 9个RFLP及SSR分子标记构建的连锁图和STATISTIC等分析软件 ,以单株结实率作为耐冷性评价指标。分析结果显示单株结实率与单株特定颖花结实率之间的相关系数 (r)为 0 .836 4 ;与耐冷性 (以单株结实率为指标 )相关的分子标记有 4 3个 ,分布在8条染色体的 11个区域。与单株特定颖花结实率相关分子标记有 34个 ,分布在 7条染色体的 9个区域 ,这 7条染色体属于与耐冷性相关的 8条染色体之中 ;与单株特定结实率相关的这 9个区域均属于与耐冷性相关 11个区域之中。表明用特定颖花结实率可以很好地反映植株受冷害情况 ,单株特定颖花结实率可以作为耐冷性鉴定指标。最后就特定颖花结实率来替代整穗结实率问题展开了讨论

【Abstract】 Here is the third part of a series papers to describe the studies on cold tolerance of rice. This part describes the results obtained by using the parents, Kunmingxiaobaigu, on indigenous variety with cold-tolerance from Yunnan, and Towada, a cold-sensitive variety bred in Japan, as well as their F 2 progenies as tested materials, combining by using 159 RFLP and SSR markers and STATISTIC software. The total fertility percentage (TFP) was used as indicator of cold tolerance. The coefficient of relationship between TFP and special fertility percentage(SFP) of single plant was 0.8364. 43 markers were related to cold tolerance based on the TFP as its indicator, distributed in eleven regions on eight chromosomes while 34 ones related to SFP over nine regions on seven chromosomes. The seven chromosomes related to SFP belonged to the eight ones related to TFP. The nine regions related to SFP belonged to the eleven regions related to TFP. The above results showed that SFP can be used to represent TFP in evaluation of cold tolerance. The issue over SFP to represent TFP in other fields was discussed.

【基金】 云南省自然科学基金;跨世纪人才基金;作物遗传改良国家重点实验室基金资助
  • 【文献出处】 作物学报 ,Acta Agronomica Sinica , 编辑部邮箱 ,2003年05期
  • 【分类号】S511
  • 【被引频次】37
  • 【下载频次】266
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