【摘要】 利用花粉管通道法 ,再次将大豆 DNA导入在生产上具有应用前景的高产粳稻 97冬繁 10 ;测定了在常规施肥基础上 ,后期追施尿素氮肥、整个生育时期只施有机肥和常规施肥 3种施肥方式下种植的 97冬繁 10、导入大豆 DNA后的 97冬繁 10、97冬繁 13和优丰 4种水稻的糙米蛋白质含量。结果显示 ,1在高氮、有机和常规 3种施肥方式下 ,已导入大豆 DNA的 97冬繁 10水稻糙米蛋白质平均含量依次为 12 8.0 ,12 3.7和 10 2 .1m g/ g,分别比相同条件下对照提高 4 7.3,4 8.0和 33.3m g/ g;2虽然高氮和有机栽培的稻米蛋白质平均含量都比常规栽培稻米高 ,但 4种水稻糙米蛋白质平均含量增加程度及变异范围不同。经大豆 DNA处理的水稻在高氮和有机栽培条件下 ,糙米蛋白质平均含量增加最明显 ,而且变异范围明显加宽。本研究结果进一步说明 ,利用花粉管通道法导入大豆 DNA能够提高水稻糙米蛋白质含量 ,并且后期追施氮肥和早期施用有机肥栽培 ,更有利于其糙米蛋白质含量的表达。更多还原

【Abstract】 The exogenous DNA from soybean was introduced into rice seeds through the pollen tube pathway again.Three kinds of culture ways were designed:the culture in high nitrogenous fertilizer,the culture in organic fertilizer,the culture in conventional fertilizer.Four kinds of rice-two ordinary varieties,the variety induced by exogenous DNA from soybean and the contrast were respectively planted with the three kinds of culture ways.Crude protein contents in the four kinds of rice offsprings seeds were analyzed.The results indicated:(1) The average contents of crude protein in the seeds induced by exogenous DNA from soybean and planted in the high nitrogenous fertilizer,in the organic fertilizer and conventional culture were 128.0 mg/g,123.7 mg/g and 102.1 mg/g,increasing separately by 473.0 mg/g,479.7 mg/g and 332.9 mg/g over their contrasts planted in relevant soils;(2) Variable range of crude protein contents in the variation rice seeds was wider in high nitrogenous fertilizer culture and in organic fertilizer culture than in the conventional fertilizer.This research result confirms that introducing exogenous DNA through the pollen tube pathway could increase crude protein content in rice seeds.The culture ways in high nitrogenous fertilizer and in organic fertilizer are advantageous to expression of crude protein content in the variation rice seeds.更多还原