节点文献
小麦遗传转化中潮霉素适宜筛选浓度的研究
Study on the optimal mass concentration of hygromycin in wheat transformation
【摘要】 研究了潮霉素不同质量浓度对西农 1376和京花 1号小麦愈伤组织及种子的筛选效果。结果表明 ,潮霉素对小麦愈伤组织的适宜筛选质量浓度为 110 mg/L,对转基因小麦后代种子筛选以 14 0 mg/L 较为适宜 ;不同基因型材料对潮霉素的敏感性存在一定差异
【Abstract】 The selection effects on the wheat immature embryo calli and the seed of Xinong 1376 and Jinghua 1 with different mass concentration of hygromycin were studied.The results showed that the optimal mass concentration of hygromycin was 110 mg/L to wheat immature embryo calli selection and 140 mg/L to wheat seed.There was a little difference of different wheat genotype to hygromycin.
【基金】 国家重点科技 (攻关 )项目 (96-0 0 9-0 1-14 ) ;华中农业大学作物遗传改良国家重点实验室开放课题 (2 0 0 0 -3 6)
- 【文献出处】 西北农林科技大学学报(自然科学版) ,Journal of Northwest Sci-Tech University of Agriculture and Forestry , 编辑部邮箱 ,2003年01期
- 【分类号】S512.1
- 【被引频次】50
- 【下载频次】569