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SSR鉴定SC704玉米亲本及杂交种纯度的研究
Assessment on Identification the Purity of Maize Hybrid SC704 and Its Parents by Using SSR
【摘要】 从30对SSR随机引物中筛选确定了能够清楚、稳定、准确的鉴别SC704亲本和杂交种的2对引物。用引物之一phi053对人为混杂有SC704亲本的杂交种进行纯度鉴定,结果准确无误。探讨并建立了一套应用分子标记SSR技术鉴定SC704玉米亲本及杂交种纯度的方法。与田间种植鉴定相比较,SSR技术用于检验玉米品种的纯度是可靠可行的。
【Abstract】 Two pairs primers from thirty pairs of primers can identify distinctly the hybrid sC704 and its parents. Primer phi053 was selected for SSR identification with hybrid SC704 blending a few parents seeds. Correct and reliable result was gained. A rapid and accurate identification system of Hybrids SC704 and its parents was established. Comparing with cultivation verification,it is feasible and reliable that SSR was used to identify maize hybrid purity.
【基金】 北京市自然科学基金(H030730010690);"玉米新品种DNA指纹图谱数据库"资助。
- 【文献出处】 西北农业学报 ,Acta Agriculturae Boreali-occidentalis Sinica , 编辑部邮箱 ,2003年04期
- 【分类号】S513
- 【被引频次】42
- 【下载频次】164