【摘要】 目的 :应用本室制备的抗单链DNA (ssDNA)单克隆抗体(mAb) ,检测多种化疗药物对不同肿瘤细胞株凋亡的诱导作用 ,并根据凋亡率判断肿瘤细胞对化疗药物的敏感性。方法 :用血浆峰值浓度 (PPC)的 9种化疗药物 ,分别诱导肿瘤细胞株K5 6 2和Hep 2的凋亡。用抗ssDNAmAb免疫组化染色法 ,检测肿瘤细胞株的凋亡并计算调亡率。结果 :以抗ssDNAmAb建立的检测细胞凋亡的免疫组化法 ,能够区分凋亡细胞和非凋亡细胞。该法检测证明 ,化疗药物可诱导敏感肿瘤细胞株凋亡 ,但不同化疗药物诱导同一肿瘤细胞株或不同肿瘤细胞株凋亡的程度不同。结论 :抗ssDNAmAb免疫组化法 ,可用于检测肿瘤细胞对化疗药物的敏感性。更多还原

【Abstract】 AIM: To explore the possibility of application of self made anti ssDNA mAb to detecting the apoptosis of tumor cells induced by chemotherapeutic drugs and evaluate the sensitivity of tumor cells to chemotherapeutic drugs from a apoptotic rate. METHODS: Apoptosis of both K562 and Hep 2 cells were induced by maximum plasma concentrations of 9 different chemotherapeutic drugs. The apoptosis of tumor cells was detected by immunohistochemical (IHC) staining with anti ssDNA mAb and their apoptotic rate were calculated. RESULTS: The apoptotic cells could be differentiated from non apoptotic cells by the HIC staining with anti ssDNA mAb.The apoptosis of tumor cells were induced by chemotherapeutic drugs, but apoptotic extent of different tumor cells, even the same one tumor cell, was distinct when they exposed to all kinds of anti tumor drugs. CONCLUSION: The combination of anti ssDNA mAb with IHC staining can be used to detect the sensitivity of tumor cells to chemotherapeutic drugs.更多还原