【摘要】 目的 :检测人缺血脑组织中TNF α和IL 1β的表达。 方法 :将 13例脑梗死的死亡病例按发病时间分成 <2d、3～ 5d、 >5d3个组 ,以非缺血侧半球作为对照 ,用免疫组化染色法检测缺血脑组织中TNF α和IL 1β的表达。结果 :人脑缺血后缺血病灶中TNF α和IL 1β呈高表达 ,与正常侧脑组织相比较有极显著差异。TNF α和IL 1β表达细胞的分布与缺血灶相一致 ,呈局灶性分布。TNF α的表达高峰在病后 2d内。IL 1β表达高峰在病后 3～ 5d。在病后 5d ,缺血侧TNF α和IL 1β的表达与正常侧无显著差异。结论 :人类脑缺血组织中TNF α和IL 1β的表达与动物实验的结果相似 ,提示TNF α和IL 1β参与了脑缺血损伤 ,有助于临床建立新的有针对性的治疗措施更多还原

【Abstract】 AIM: To detect the expression of TNF α and IL 1β in human cerebral ischemic tissues. METHODS: 13 cerebral specimens from patients died of cerebral infarction were divided into three groups, <2 d, 3～5 d, and >5 d, according to the lasting time of infarctions. The expression of TNF α and IL 1β in the cerebral ischemic tissues were examined by immnohistochemical staining. The contralateral tissues were employed as controls. RESULTS: The expression of TNF α and IL 1β in the cerebral ischemic tissues were significant higher than those in the contralateral tissues. The focal distribution of the TNF α + and IL 1β + cells was identical with the ischemic area. The expression of IL 1β and TNF α peaked at the 3rd to 5th and 2nd day after ichemia, respectively. There were no significant difference between the ischemic and contralateral brains at the 5th day after ischemia for the expression of TNF α and IL 1β . CONCLUSION: Our results showed the expression of TNF α and IL 1β in human strok of infarction were similar to those in animal experiments. It is suggested that TNF α and IL 1β are involved in cerebral ischemic injury, which will be helpful for developing clinically a novel therapy aiming at cerebral ischemic injury.更多还原