【摘要】 目的 :探讨人内皮细胞中一种新的可溶型黏附分子sCD2 2 6的表达调变规律。方法 :用ELISA方法 ,检测不同浓度的LPS刺激人脐静脉内皮细胞后不同时间点培养上清中sCD2 2 6含量的变化 ;用Greiss法检测上述细胞培养上清中NO含量的变化 ,并分析两者的线性相关性。加入抗TNF α中和抗体和iNOS抑制剂后 ,观察其对LPS诱导sCD2 2 6生成的影响。结果 :①随着LPS刺激剂量的增加和刺激时间的延长 ,HUVEC培养上清中sCD2 2 6的含量明显增加 ,10 0mg/LLPS刺激 3d后sCD2 2 6的含量为 188.5 μg/L。②LPS刺激剂量为10 0mg/L时 ,HUVEC培养上清中sCD2 2 6和NO的含量呈显著的正相关。③抗TNF α中和抗体和iNOS抑制剂可显著抑制LPS活化的HUVEC产生sCD2 2 6。结论 :LPS可诱导HUVEC产生sCD2 2 6分子 ,且sCD2 2 6的生成与NO和TNF α的产生密切相关更多还原

【Abstract】 AIM: To explore the expression and regulation of a novel soluble adhesive molecule sCD226 on HUVECs. METHODS: The concents of sCD226 and NO in culture supernatant of LPS activated human umbilical vein endothelial cells (HUVECs) were detected by ELISA and Greiss method respectively , and linear correlativity of contents between sCD226 and NO was analysed. The influence of anti TNF α mAb and iNOS inhibitor on LPS induced sCD226 production was observed by ELISA. RESULTS: ①LPS might markedly stimulate sCD226 production from HUVECs in a dose and time dependent manner. The amount of sCD226 came to 188.5 μg/L when endothelial cells were stimulated by 100 mg/L LPS for 3 days. ②The production of NO and sCD226 showed linear correlation when HUVECs were stimulated by 100 mg/L LPS. ③LPS induced sCD226 production in HUVECs was inhibited significantly by anti TNF α mAb and iNOS inhibitor. CONCLUSION: LPS activated HUVECs can secret sCD226 and the generation of sCD226 is closely correlated with the production of NO and TNF α.更多还原