【摘要】 利用Plackett-Burman设计筛选出影响盐生盐杆菌(Halobacteriumhalobium)B培养条件的重要因素,然后利用正交试验设计对这些重要因素加以优化。结果表明,盐生盐杆菌B的最适培养条件为:MgSO4·7H2O20g/L,酵母膏10g/L,KCl2.5g/L,FeSO4·7H2O50mg/L,NaCl200g/L,柠檬酸钠2g/L,酪蛋白氨基酸5g/L,甘油10g/L,pH6.5,温度38℃,摇瓶转速180r/min,光照培养。更多还原

【Abstract】 The important fators which affect culture conditions of Halobacterium halobium B were screened by PlackettBurman design.Then by using orthogonal test,these important factors were optimized.Results showed that the best culture conditions of Halobacterium halobium B were MgSO4·7H2O,20 g/L;Yeast extract,10 g/L;KCl,2.5 g/L;FeSO4·7H2O,50 mg/L;NaCl,200 g/L;tyigodium citrate,2 g/L;casamino acid,5 g/L;glycerol,10 g/L;pH,6.5;temperature,38℃;shake speed,180 r/min and illumination.更多还原