【摘要】 目的　研究国产褪黑素对人食道磷癌Eca 10 9细胞的生长抑制作用及其作用机理。方法　采用不同浓度的褪黑素通过体外细胞培养、MTT显色技术研究其抑制作用 ,通过流式细胞仪和透射电子显微镜技术观察 ,对其作用机理进行探讨。结果　褪黑素对人食道磷癌Eca 10 9细胞具有抑制作用 ,其IC30 、IC50 分别为 0 .89、1.6 0mmol·L- 1 。以IC30 的褪黑素处理Eca 10 9细胞 ,使其倍增时间由 33.6h延长到 39.4h。流式细胞仪观察到褪黑素可导致Eca 10 9细胞G0 G1 期比例升高 ,S期比例降低。透射电镜可观察到以IC30 的褪黑素处理的细胞 ,其超微结构发生了退行性改变。结论　褪黑素对人食道磷癌Eca 10 9细胞具有抑制作用 ,其作用机理可能是褪黑素导致细胞周期从G1 到S阶段的推迟进行更多还原

【Abstract】 Objective To study the inhibitory effect of melatonin on the growth of Eca-109 cells of human esophageal squamous cell carcinoma Eca-109 line and its inhibitory mechanism. Methods The inhibitory effects of melatonin with various concentrations on the human squamous cell carcinoma Eca-109 line in vitro were determined with MTT method. The inhibitory mechanism was investigated by flow cytometry (FCM) and transmission electron microscopy (TEM). Results The IC 30 value and IC 50 value were 0.89?mmol·L -1 and 1.60?mmol·L -1, respectively. The population doubling time of Eca-109 cells treated with melatonin at 0.89?mmol·L -1 was 39.4?h, as compared with 33.6?h of the control group. Under FCM we found that the cell was inhibited by melatonin during phase G 0/G 1 and the cell percentage during stage S decreased. The degenerated ultra-structure of the cell treated with melatonin was also observed by TEM. Conclusion The results suggest that melatonin can inhibit the growth of Eca-109 cells in human squamous cell carcinoma. The mechanism of inhibition might be the prolonging of the staging from G 1 to S in the cell cycle.更多还原