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降脂中药对爪蟾卵母细胞存活率的影响

Influence of Chinese traditional medicines depressing lipid on the livability of Xenopos oocyte

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【作者】 罗艳钱颖范春雷严瑾高丽萍窦小兵

【Author】 LUO Yan QIAN Ying, FAN Chun-lei, et al. Department of Biochemistry of Medical College, Hangzhou leathers College, Hangzhou, Zhejiang 310012

【机构】 杭州师范学院医学院生物化学教研室浙江中医学院生命科学系浙江中医学院生命科学系 浙江 杭州 310012浙江 杭州 310053浙江 杭州 310012浙江 杭州 310053

【摘要】 目的:探讨降脂中药对爪蟾卵母细胞存活率的影响。方法:用含降脂中药的培养液培养爪蟾卵母细胞,以孕酮成熟实验计算细胞的存活率。结果:用药组的存活率达到90%的培养液浓度为:山楂酸提物0.025mg/ml,川芎嗪0.4mg/ml,姜黄醇提物6.45×10-3mg/ml。结论:姜黄醇提物、山楂醇提物、川芎嗪等降脂中药可在上述用药浓度范围内培养爪蟾卵母细胞。

【Abstract】 Objective: To explore the influence of Chinese traditional medicines depressed lipid on the livahility of Xenopus oocyte. Methods: Xenopus oocyte was cultivated in the culture fluids containing Chinese traditional medicines and the livabilily of Xenopus oocyte was calculated under the test of its maturity with progesterone. Results: Concentrations of the culture fluids of the group in which the livability reached 90% by using Chinese traditional medicines are: 0.025 mg/ ml (alcohol extract of hawthorn), 0.4 mg/ml (rhizome of Sichuan lovage), and 6.45 × 10-3(alcohol extract of curcum-a). Conclusion: Chinese traditional medicines depressed lipid, such as hawthorn, rhizome of Sichuan lovage and curcuma can cultivate Xenopus oocyte with the concentrations mentioned-above.

【基金】 国家自然科学基金(批准号:30070932)
  • 【文献出处】 温州医学院学报 ,Journal of Wenzhou Medical College , 编辑部邮箱 ,2003年03期
  • 【分类号】R285
  • 【下载频次】52
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