【摘要】 以大麦、小麦品种为材料 ,分别采用醇溶蛋白提取剂乙醇、乙二醇、2 -氯乙醇、2 -巯基乙醇、尿素等配成不同浓度的单一组分提取剂和复合提取剂 ,在同一条件下提取大麦和小麦去胚种子的醇溶蛋白 ,通过 A- PAGE分离样品液发现 ,单一组分提取剂中 2 5 %的 2 -氯乙醇效果较好 ,5 %的 2 -巯基乙醇效果较差 ;复合提取剂中由 2 -氯乙醇和2 -巯基乙醇组成的提取剂效果最好。这种复合提取剂制备的样品液经电泳后 ,在图谱中条带丰富 ,带型清晰 ,无论对大麦还是小麦种子 ,都是最佳的醇溶蛋白提取剂。更多还原

【Abstract】 The alcohol-soluble proteins (hordeins from barley, gliadins from wheat) were extracted from the embryo-removed seeds of four barley cultivars (Hordeum vulgare L.) and three wheat cultivars (Triticum aestivum L.) with different extracting solvents. The results revealed that the extraction of hordeins and gliadins was most efficient with extraction solution composed of the 2-chloroethanol (25%) and 2-mercaptothanol (1%). Using this extractant, more hordein or gliadin protein bands could be resolved by polyacrylamide gel electrophoresis under acid pH condition.更多还原