【摘要】 通过正交试验,结果表明枯草芽孢生防菌TH2高产抗菌物质培养基的最佳配方为豆饼粉1.5％,玉米粉为0.5％,麦麸0.5％,大米粉0.5％,最佳的培养条件为通气量25ml装瓶量、pH为7.5、温度为28±1℃,这样产生的抗菌物质最多。枯草芽孢生防菌株TH₂培养过程中,pH从培养初始的7.0,经下降又逐步上升到48小时的7.8。OD650从培养初始的0.56,逐步上升到36小时的1.10,而后逐渐下降,84小时降到0.85。孢子生成从12小时开始,孢子数达（108cfu/ml）,而后逐渐增加,48小时达到高峰,孢子数达58.2（108cfu/ml）,随后孢子数逐渐下降,抑菌圈值随着发酵时间增加逐渐增大。更多还原

【Abstract】 The present paper dealt with selection of fermentation conditions for the growth of the antibiotic—producing bacterium strain TH₂ （Bacillus subtilis）. The results showed that the optimal cultural media consisting of 1.5% bean cake powder, 0.5% corn flour, 0. 5% peptone, according the fermentation test, on which the optimal conditions for the fermentation of strain TH₂ were pH 7.5 and temperature28±1℃. Like this, anti—fungal—bacterium substance is at most. During the fermentation, pH gradually decreased down from 7.0 at the beginning then increased up to 7.8 at 48 hours. OD₆₅₀ raised up from 0.56 at the beginning to 1.10 at 36 hours then decreased down to 0.85 at 84 hours. The peak value of spore generation was 58.2 （10⁸cfu/ml） at 48 hours. Inhibition zone along with ferment the time gain to gradually enlarge.更多还原