【摘要】 以可溶性淀粉为底物配制固体培养基,然后低温处理(4℃)平板培养物,产淀粉酶的菌株其菌落周围的培养基由白色变成灰色且透明.该方法不具有破坏性,不需要预先接种菌落,能直接发现和分离分解利用淀粉的微生物.利用淀粉琼脂平板还能快速验证淀粉酶的耐酸性,根据在pH4.2和pH6.0的琼脂平板上透明圈的直径d与淀粉酶活力的对数lnA的关系,求出淀粉酶在pH4.2和pH6.0时的淀粉酶活力.pH4.2时比pH6.0时的比值大,说明该淀粉酶产生菌的耐酸性较强.更多还原

【Abstract】 A simple and rapid method was proposed for the detection of amylaseproducing microorganisms on solid media using soluble starch as substrate. The soluble starch was incorporated into nutrient agar and amylase production could be detected when the color of the white media became grey around the colonies. The method was nondestructive, and allowed direct visualization and isolation of amylolytic microorganisms from the environment without prior replication of colonies. From the relationship of d with lnA,the amylase activity at pH 4.2 and pH 6.0 was determined. The acidtolerance ability could be easily testified by comparing amylase activity at pH 4.2 and pH 6.0,at starch agar plate.更多还原