【摘要】 目的 :为提高心肌梗死的早期诊断水平 ,本文探讨用脑肌酸激酶单克隆抗体酶联免疫吸附法对血清肌酸激酶B亚基含量进行测定。方法 :用BCK McAbELISA法测定B亚基含量 ,动力学法测定CK、CK MB的活性 ,并对结果进行动态观测及比较。结果 :血清CK B亚基含量在心肌梗死急性发作期为 344 .89± 2 0 5 .18ng/ 5 0 μl;正常对照组和缓解期B亚基分别为 6 .5× 10 - 1 ± 2 .2× 10 - 4 ng/ 5 0 μl和 18.5 0× 10 - 4 ± 12 .2 0× 10 - 4 ng/ 5 0 μl。血清B亚基在心肌梗死急性发作期、缓解期、对照组中含量变化有显著性差异 (P <0 .0 5 )。结论 :BCK McAbELISA法可检测血清中微量B亚基。当心肌梗死急性发作 ,CK、CK MB尚在正常范围时 ,B亚基含量已明显升高。使用本法测定血清B亚基含量具有准确、快速、灵敏、可靠、特异性高等特点 ,为临床上早期诊断急性心肌梗死和疗效判断提供较好的方法更多还原

【Abstract】 Objective: In order to improve the clinical diagnosis of Acute myocardial infarction,in this paper investigates the subunit of CK McAb ELISA with CK B to test the content of CK B.Method: Detected the concentration of CK B subunit by ELISA and to assay activity of CK MB by the onethod of dynamies.Results: Both the acute period in AMI was 344.89±205.18 ng/50 /μl and the relieved period was 18.50×10 -4 ±12.20×10 -4 ng/50 μl,that were are higher than the nomat control group (6.5×10 -1 ±2.2×10 -4 ng/50 μl) it was significant differences( P<0.05 ).Conclusion: This method for Bck McAb ELISA is an effective and predise method for detection of serum CK B during AMI in acute period also CK,CK MB delayed in a normol rauge,that CK B had been increased obviously,this method is a good way for clinical diagnose on AMI.更多还原