【摘要】 为探讨膳食钙对高脂膳食大鼠骨骼肌解偶联蛋白 3 (UCP3 )基因表达的影响 ,将雄性Wistar大鼠随机分成 6组 ,每组 9只 ,分别喂以基础饲料A组 ( 0 5 %钙 )、高脂低钙饲料B组 ( 0 0 8%钙 )、高脂正常钙饲料C组 ( 0 5 %钙 )、高脂高钙饲料D和E组 ( 1%和 1 5 %钙 )和高脂高钙 ( 1 5 %钙 ) +维生素D( 60 0IU % )饲料F组 ,9周后取血测全血血糖、血清瘦素、胰岛素、甲状腺激素 ,应用RT PCR方法测定骨骼肌UCP3mRNA表达水平。结果显示 ,C组体重和体脂含量明显高于A组 (P <0 0 5 ) ,膳食高脂高钙的D、E、F组体重增加的趋势明显降低。C组血糖和胰岛素均有增高趋势 ,C组胰岛素显著高于A、D、E、F组 (P <0 0 5 ) ,但血糖各组无显著性差异 (P >0 0 5 )。与A组相比 ,C组血清瘦素水平显著增高 (P <0 0 5 ) ,D、E、F组血清瘦素有增高趋势 ,但无显著性差异 (P >0 0 5 )。D、E、F组血清游离三碘甲腺原氨酸 (FT3 )明显增高 ,与A、B、C组相比均有极显著性差异 (P <0 0 1)。血清游离四碘甲腺原氨酸 (FT4)各组无显著性差异 (P >0 0 5 )。高脂正常钙的C组骨骼肌UCP3mRNA水平明显低于基础组 ,高脂高钙的D、E、F组UCP3mRNA与C组相比均明显增强。表明膳食钙不但可以改善高脂膳食诱发的体重增加的趋势 ,改善高胰岛素血症 ,还更多还原

【Abstract】 In order to study the effects of dietary calcium on the expression of uncoupling protein3(UCP3) in skeletal muscle of Wistar rat fed with high fat diet, 54 male Wistar rats were randomly divided into 6 groups: Group A was fed with basic diet(0.5%calcium),Group B was fed with high fat low calcium diet(0.08%calcium),Group C was fed with high fat normal calcium diet(0.5%calcium),Group D and E was fed with high fat high calcium diet(1% and1.5% calcium respectively),Group F was fed with high fat high calcium diet (1.5% calcium)supplemented with vitamin D(600IU%). Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the level of UCP3 mRNA. The results showed that the body weight and fat content in group C were significantly higher than those in group A. However, the body weight gain in groups of D, E, and F was less than those in group C. The serum insulin in group C increased significantly compared with that in groups of A,D,E and F(P<0.05),but there was no significant difference in blood glucose among all groups. Compared with group A, the level of serum leptin increased significantly in group C(P<0.05)and increased slightly in groups D,E and F (P>0.05).The level of serum FT3 in groups D,E and F increased significantly compared with that of group A,B and C(P<0.05),but there was no significant difference in FT4 among all groups(P>0.05). Compared with group A, the expression of UCP3 mRNA decreased in skeletal muscle in group C, while UCP3 mRNA increased in groups D,E and F compared with that of group C. In conclusion, dietary calcium may not only reduce the body weight gain induced by high fat diet, and improve hyperinsulinemia, but also may enhance the expression of UCP3 mRNA in skeletal muscle by increasing the serum level of leptin and FT3 which may play the role in the prevention of obesity.更多还原