【摘要】 利用生物信息学方法和工具开发了微生物基因组注释系统 (Microbialgenomeannota tionpackage ,MGAP) ,并用于蓝细菌PCC70 0 2的基因组注释。该系统由基因组注释系统和基于Web的用户接口程序两部分组成。基因组注释系统整合多个基因识别、功能预测和序列分析软件 ;以及蛋白质序列数据库、蛋白质资源信息系统和直系同源蛋白质家族数据库等。用户接口程序包括基因组环状图展示、基因和开放读码框在染色体上的分布图 ,以及注释信息检索工具。该系统基于PC微机和Linux操作系统 ,用MySQL作数据库管理系统、用Apache作Web服务器程序 ,用Perl脚本语言编写应用程序接口 ,上述软件均可免费获得。更多还原

【Abstract】 A Microbe Genome Annotation Platform (MGAP) was developed and applied to the cynobacterium PCC7002 genome annotation. Various bioinformatics software tools from sequence analysis to gene identification and function prediction were implemented in MGAP. Protein sequence databases SWISSPROT and PDBseq, protein information resource InterPro and COG were also integrated in the platform. The web interface of MGAP has the functionality to display a circular map of gene distribution and GC contents throughout the genome. Detailed information such as the DNA and protein sequence, the location of genes on chromosomes can be viewed by clicking the corresponding object within the map. MGAP is based on a PC/Linux system affordable for small biological laboratories and has the advantage of using free software tools including MySQL, Apache and Perl.更多还原