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生孢噬纤维细菌的滤纸纤维素的降解过程研究
Study on the Process of Degrading Filter Paper Cellulose by Sporocytophaga sp. JL-01
【摘要】 生孢噬纤维细菌 (Sporocytophaga)是能够降解纤维素的滑动细菌 ,它可将滤纸和棉花纤维素完全降解 ;但其可测得的纤维素酶活性极低。为研究其纤维素降解机制 ,本文采用扫描电子显微镜观察了一株生孢噬纤维细菌 (Sporocytophagasp .)JL 0 1对滤纸纤维素的降解过程 ,分析了在此过程中滤纸纤维素的降解与菌体形态变化之间的关系。结果表明 :生孢噬纤维细菌在纤维素降解过程中 ,形成长度为 2 5 μm~ 4 0 μm可弯曲的细长杆状细胞 ,该细长杆状细胞通过与纤维素分子的吸附或嵌入纤维素中完成对纤维素的降解 ;在降解后期 ,杆状细胞转化为直径约为 0 6μm的小孢囊休眠细胞。
【Abstract】 Sporocytophaga is a kind of bacteria that can glide on the solid medium surface. It can decompose the cotton and filter paper after 6 days cultured. The sporocytophaga can only produce a low extracellular carboxymethylcellulase activity and no other cellulase activities. In this work, the process about degradation of filter paper fibre by a strain of Sporocytophaga was studied using scan electronmicroscopy. The morphological changes that Sporocytophaga sp. Jl-01 grew on filter paper were observed. The study revealed the JL-01 adhered tightly to the surface of filter paper fibre or penetrated into interior of the fibre through its 2.5μm~4.0μm bacilliform cells in the process of degrading. The bacilliform cells degrade the cellulose strongly and produced a mass of sticky polysaccharides. At the anaphase of cultured, the bacteria existed as a cycloidal dormancy body-sporocyst.
【Key words】 Sporocytophaga; Bacilliform cell; Cellulose degrading; Sporocyst;
- 【文献出处】 微生物学报 ,Acta Microbiologica Sinica , 编辑部邮箱 ,2003年06期
- 【分类号】Q935
- 【被引频次】28
- 【下载频次】405