【摘要】 研究核定位信号对真核化的T7表达系统在真核细胞及基因免疫中表达HBsAg基因效率的影响。结果发现 ,在体外培养细胞中 ,无核定位信号的T7系统表达效率比有核定位信号组明显要高。基因免疫时 ,无核定位信号组诱发小鼠产生了针对HBsAg的特异性免疫应答 ,而含核定位信号的T7表达系统则未能诱发小鼠发生明显的免疫应答。提示T7系统是一种胞浆表达系统 ,将T7RNA聚合酶引入核定位信号后该系统的表达效率降低 ,甚至不能诱发特异性免疫应答。更多还原

【Abstract】 To investigate the effects of nuclear localization signal on immune response induced by eukaryonized T7 expression system. Nuclear localization signal (NLS) coding sequence was introduced into eukaryonized T7 expression system containing HBV M protein coding gene. Naked gene immunization was performed. The results showed that the expression efficiency of T7 system which containing nuclear localization signal in vitro was lower than that without NLS. In vivo study found that HBV specific immune response induced by NLS lacking eukaryonized T7 system was significantly higher than that in eukaryonized T7 expression system containing NLS. HBV specific Th1 and Th2 type immune responses were also observed in the mice immunized with T7-HBsAg plasmid lacking NLS, indicating that the nuclear localization signal might not be essential in T7 expression system.更多还原