节点文献
杏鲍菇抗烟草花叶病毒蛋白的筛选
Screening of Proteins Anti |Tobacco Mosaic Virus in Pleurotus eryngii
【摘要】 采用离子交换层析和凝胶层析方法 ,从杏鲍菇干样中分离得到多个蛋白组分 ,经枯斑寄主检测 ,发现多个蛋白组分都有抗烟草花叶病毒 (TMV)的活性 ,对TMV的抑制率均在 70 %以上 ,高者可达 99%。其中xb68Ab已得到了纯化 ,分子量约为 2 3 7kD ,在心叶烟和苋色藜上它对TMV侵染的抑制率分别达到 99 43%和 98 9%。
【Abstract】 Proteins were obtained by ion |exchange chromatography and gel filtration from the dry sample of %Pleurotus eryngii. % In terms of detection on local lesion host, these components were confirmed to be anti |TMV and their inhibition rates reach more than 70%, or even 99%. The protein named xb68Ab of MW 23 ^7kD was purified by ion |exchange chromatography and gel filtration. its inhibition rate against TMV are 99 ^43% and 98 ^9% on %Nicotiana glutionosa % and %Chenopodium amaranticolor %, respectively.
【基金】 福建省科技厅重点项目 ( 99-Z1- 93)
- 【文献出处】 微生物学报 ,Acta Microbiologica Sinica , 编辑部邮箱 ,2003年01期
- 【分类号】S432.4
- 【被引频次】68
- 【下载频次】289