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单组分妥布霉素产生菌的快速筛选

The Method for Quick Screen Single Compound Tobramycin Producing Strain

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【作者】 夏焕章刘晓辉任丹熊宗贵

【Author】 XIA Huanzhang,LIU Xiaohui,REN Dan,XIONG Zonggui(Department of Biopharmaceutics,Shengyang Pharmaceutical University,Shengyang,110016);

【机构】 沈阳药科大学生物制药系沈阳药科大学生物制药系 辽宁沈阳110015辽宁沈阳110015辽宁沈阳110015

【摘要】 经多轮筛选获得了 1株妥布霉素耐药安普霉素敏感的芽孢杆菌。利用该菌与枯草杆菌 6 35 0 1混合作为检定菌 ,从妥布霉素和安普霉素二组分产生菌中快速筛选到了单组分妥布霉素产生菌。

【Abstract】 Bacillus TR-1 was highly resistant to tobramycin but remained sensitive to apramycin andBacillus sutilis 63501 was sensitive to tobramycin and apramycin. The two organisms were mixed and cultured to assay the relative contents of tobramycin and apramycin.Streptomyces tenebrarius 9901 which had produced tobramycin and apramycin was treated with NTG, single compound of the tobramycin producing strain was obtained using by this method.;

  • 【文献出处】 微生物学杂志 ,Journal of Microbiology , 编辑部邮箱 ,2003年01期
  • 【分类号】TQ465
  • 【被引频次】9
  • 【下载频次】126
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