【摘要】 采用前体添加实验法、静息细胞培养法以及酶抑制剂法对藤黄灰链霉菌中抗生素AG PM生物合成途径进行了初步探讨。研究表明能转化成聚酮合成所需活性前体的氨基酸如异亮氨酸、缬氨酸、蛋氨酸、谷氨酸等以及短链脂肪酸乙酸、丙酸、丁酸盐对抗生素AGPM合成均有明显促进作用 ;另外 ,在培养基中添加脂肪酸和聚酮生物合成途径的专一性抑制剂浅蓝菌素 (2 5μg mL)或脂肪酸合成抑制剂碘乙酰胺 (0 5mmol L)时 ,菌体生长不受影响 ,而抗生素AGPM合成受到强烈抑制 ,分别为对照的 35 3 %和 2 6 2 % ;当碘乙酰胺的浓度为 1mmol L时 ,抗生素AGPM合成几乎完全受到抑制。由此初步推断抗生素AGPM是由聚酮途径合成。更多还原

【Abstract】 A new antitumor antibiotic AGPM biosynthesis pathways was studied with feeding e xperiment, resting cell system and enzyme inhibitor experiments. The results showed that addition of the amino acids and branched fatty acids such as Ile, Val, Met, Glu and acetate, propionate and butyrate, which could be transfo rmed to active precursors of polyketide, could effectively improve the productio n of antibiotic AGPM. Cerulenin(25μg/mL) and iodoacetamide (0.5mmol/L)-s peci fic inhibitors of polyketide synthase and fatty acids synthase could decrease an tibiotic production to 35.3% and 26.2% that of control respectively, without a ffecting cell growth. When idioacetamide concentration increased to 1.0mmol/L, antibiotic AGPM production would be fully inhibited. All these results testifi ed antibiotic AGPM was biosynthesized by polyketide pathway.更多还原