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用指纹图谱评价环境样本DNA的提取效果
EVALUATION ON DNA EXTRACTION OF ENVIRONMENTAL SAMPLES BY DNA FINGERPRINTS
【摘要】 研究了DNA指纹图谱技术在评价环境样本DNA提取效果方面的应用。采用 3种DNA提取方法提取垃圾渗滤水和活性污泥中的微生物DNA ,并用ARDRA和RISA图谱加以评价。实验结果表明 ,RISA图谱分析法可以作为评估DNA提取效果的有效手段
【Abstract】 In this paper the application of DNA fingerprints to evaluate the efficiency of DNA extraction was studied DNA mixtures of landfill leachate and active sludg e were extracted using three different methods and the DNA extraction was evalua ted by ARDRA and RISA fingerprints The results showed that RISA is a effecti ve way to evaluate DNA extraction
【关键词】 DNA提取;
微生物多样性;
16SrDNA;
ARDRA;
RISA;
【Key words】 DNA extraction; Microbial diversity; 16S rDNA; AR DRA; RISA;
【Key words】 DNA extraction; Microbial diversity; 16S rDNA; AR DRA; RISA;
【基金】 国家自然科学基金资助项目 (No. 50 2 780 53)~~
- 【文献出处】 微生物学通报 ,Microbiology , 编辑部邮箱 ,2003年04期
- 【分类号】Q789
- 【被引频次】7
- 【下载频次】208