【摘要】 在随机引物建库的基础上 ,通过交叉设计引物及加单链DNA接头 ,应用RT PCR技术克隆了家蚕质多角体病毒dsRNA片段Ⅳ的全长cDNA ,并测定了它的全序列。该片段长 3,2 6 2bp ,含有一个完整的开放读码框 ,编码一个长 1 ,0 5 8氨基酸的成熟多肽。序列分析表明 ,该片段与日本BmCPV片段Ⅳ的核苷酸序列同源性为 89% ,氨基酸序列同源性为 95 %。更多还原

【Abstract】 Based on the preparation of cDNA library using random oligonucleotide, a series of primers overlapping the whole segment Ⅳ of BmCPV and an adaptor of ssDNA were designed BmCPV dsRNA segment Ⅳ was acquired by RT PCR amplification and sequenced Segment Ⅳ was 3,262 bp long and had a single,long open reading frame BmCPV segment Ⅳ encoded a polypeptide with 1,058 amino acids Sequences analyses showed that it was homologous 89% and 95% to BmCPV dsRNA segment Ⅳ of Japan strains in nucleotides and amino acids,respectively更多还原