【摘要】 通过浊度分析和激光光散射光谱研究了二价金属离子(Ca2+、Cu2+和Mn2+)对蛋黄卵磷脂(EYPC)聚集态的影响.结果表明,自由的二价金属离子对EYPC囊泡有破坏作用,并使EYPC囊泡发生相转变,形成胶束;而与牛磺胆酸钠(TC)结合的二价金属离子使EYPC囊泡半径减小,但不破坏EYPC囊泡.更多还原

【Abstract】 It is well known that phosphatidylcholine (PC) vesicles are predomina nt carriers of cholesterol in human bile.Although the interaction of bile salts with PC has been extensively studied,relatively little attention has been given to effects of divalent metal ions on the aggregation properties of PC.We used bo th light scattering and UV spectroscopies to follow the effects of divalent meta l ions on EYPC (egg yellow PC) vesicles.We conclude that when divalent metal ion s existed,the mixed vesicles formed by PC and TC (sodium taurocholate) with the ratio of 1:3 were more stable.Free divalent metal ions act to precipitate unilam ellar PC vesicles whereas,in combination with sodium taurocholate,these ions act to decrease the size of PC vesicles and induce a phase transition from PC vesic les to mixed micelles.It is a possible reason for gallstones formation when extr a free divalent metal ions exist in human bile.更多还原