【摘要】 报导了用乙基纤维素和乙炔黑获得的导电复合材料构成的葡萄糖氧化酶生物传感器的制备方法.讨论了多种因素对该生物传感器响应电流的影响.测得此电极酶催化反应的活化能为40.3kJ·mol-1.AFM实验表明,用环己烷洗去石蜡的导电复合材料-葡萄糖氧化酶生物传感器具有粒状结构,这有利于酶催化反应的进行.更多还原

【Abstract】 This paper reports the preparation and properties of biosenor using conducting composite material consisted of ethyl cellulose and acetylene black. The final biosensor was constructed by applying a mixture containing 0.010 g ethyl cellulose, 0.004 g acetylene black,a little paraffin wax and 0.2 mg glucose oxidase (23000 units/g solid, E.C.1.1.3.4, Sigma Chemical Co.) on a platinum electrode (3 mm×3 mm). Effect of various factors on the response current of the biosensor was discussed. The response current increases linearly with increasing glucose concentration under 11.5 mmol·dm-3. Its activation energy is 40.3 kJ·mol-1 in enzyme catalyzed reaction. AFM shows that the surface of conducting polymer composite material glucose oxidase electrode′s presents uniform granular structure after paraffin wax being washed with cyclohexane, which is favorable for enzyme catalyzed reaction.更多还原