【摘要】 应用柱层析和HPLC方法从漆树漆液中分离提纯漆酶的两种同工酶L1和L2 .两者均为低温酶 ,最适的温度分别为 2 0℃和 13℃ ,pH值分别为 6.7和 7.2 ,等电点分别为 8.6和 9.1.通过十二烷基硫酸钠 聚丙烯酰胺凝胶电泳 ,测得它们的分子量分别为 1.2× 10 5和 1.0 5× 10 5.两种酶均不能催化氧化间氨基苯甲酸 ,对 3 氨基酪氨酸等有很高的反应活性更多还原

【Abstract】 Two isoforms of laccase were obtained as the main phenol-oxidases from latex of Chinese lacquer tree Rhus vernicifera. Both of L1 and L2 were low-temperature enzymes with optimum temperature 20℃ and 13℃ respectively. The laccases had very narrow optimal pH, and more stable at neutral pH than at basic pH, each with pI 8.6 and 9.1. The molecular masses of the purified laccases were about 1.2×10 5 and 1.05×10 5 by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Both of L1 and L2 can catalyze 3-amino tyrosine, but not to m-amniobenzoic acid.更多还原