【摘要】 目的　研究食管鳞癌中TGF β1的表达 ,及它在食管鳞癌细胞的增殖、侵袭、转移中的作用。方法　运用免疫组织化学方法研究TGF β1蛋白表达情况 ,显微镜下计数阳性细胞指数 (LI) ,并进行统计分析。结果　①正常食管黏膜阳性率为 2 0 % (2 / 10 ) ,食管鳞癌组织为 62 % (3 1/ 50 ) ,两者差异有显著性 (χ2 =5.93 94,P =0 .0 15) ;LI后者高于前者 (t=2 .717,P =0 .0 0 9) ,正常食管黏膜阳性 2例 ,均为弱阳性 (LI <2 5% )。②TGF β1在食管鳞癌组织中的表达与年龄、性别无明显关系 (P >0 .0 5) ,不同分化级别中两者表达亦无差异 (P >0 .0 5)。有淋巴结转移组TGF β1水平高于无淋巴结转移组 ,差异有显著性 ,P =0 .0 0 3。食管外膜浸润组TGF β1显著高于未受浸润组 ,P =0 .0 0 1,临床分期Ⅰ Ⅱ与Ⅲ Ⅳ期TGF β1表达水平差异有显著性 ,P =0 .0 0 0。结论　①食管鳞癌中存在TGF β1的高表达 ,表达水平与淋巴结转移、浸润侵袭、临床分期等有关 ,其检测有辅助诊断作用 ,且有利于判断肿瘤转移、浸润和后继治疗 ;②表达水平与组织分级无关 ,提示TGF β1在促进肿瘤局部侵袭转移中的重大作用 ,有助于理解临床上一些肿瘤生物学行为与组织分级不符的现象更多还原

【Abstract】 ObjectiveTo study the roles ofin esophage al squamous cell carcinoma(SCC).The expression of TGF-β 1 was examined in 50 cases of esophageal squamous cell carcinoma and 10 cases of normal esophageal m ucosa with SABC immunohistochemical technique.All the samples were stained by HE to confirm their pathologic characters.LI (labeling index) was evaluated by mic roscope.Categorical data were analyzed with χ 2 tests,t test,spearman rank correlation tests and logistic regression.MethodsTGF-β 1 positive expression was observed significan tly higher in esophageal SCC than in the normal esophageal mucosa (P<0.0 5).Furthermore,the over expression of this kind of protein was associated w ith late clinical stages(P<0.05),high incidence of lymph node metasta sis(P<0.05)andadventita involvement(P< 0.05) but had no thing to do with poorly differentiation (P>0.05).ResultsTGF-β 1 positive expression was observed significan tly higher in esophageal SCC than in the normal esophageal mucosa (P<0.0 5).Furthermore,the over expression of this kind of protein was associated w ith late clinical stages(P<0.05),high incidence of lymph node metast asis(P<0.05) and adventita involvement(P< 0.05) but had nothing to do with poorly differentiation (P>0.05).Conclusion TGF-β 1 is over expressed in esophageal SCC.Evaluat ing it’s LI might be useful to diagnosis and estimating prognosis.The up-regula ted TGF-β 1 correlates with lymph node metastasis,invasion and clinical stag es but has no relationship with differentation.TGF-β 1 might play an importa nt role in local invasion and metastasis of esophageal SCC cells.更多还原