【摘要】 目的　分析SLP 76基因在食管癌、贲门癌、胃癌、结肠癌及其癌旁组织中的差异表达。方法　依据微阵列技术筛选出SLP 76基因在食管癌组织中高表达的结果 ,应用逆转录 聚合酶链式反应 (RT PCR) ,对上述消化道肿瘤组织及其癌旁组织中的SLP 76基因表达进行检测。结果　SLP 76在 73 .3 % (2 2 / 3 0 )的食管癌组织中高表达 (P =0 .0 0 5) :在 75% (9/ 12 )的贲门癌组织中高表达(P =0 .0 14 ) ;在 75% (12 / 16)胃癌组织中高表达 (P =0 .0 0 5) ;在 72 .2 % (13 / 18)结肠癌组织中高表达 (P =0 .0 0 8)。结论　在RNA水平 ,SLP 76基因在上述肿瘤之间的表达无显著性差异 (P =0 .9684)。但该基因在消化道肿瘤中高表达 ,SLP 76与上述消化道肿瘤生长关系密切 ;可能具有潜在的致癌性更多还原

【Abstract】 Objective Confirm the microarray hybridization results and examine the expression of SLP-76 gene in digestive tract cancers and adjacent normal mucosa.MethodsAccording to analysis results of microarray, distinctly differential ly expressed SLP-76 gene was screening out. RT-PCR was applied to check the expression o f SLP-76 in digestive tract cancers and adjacent normal mucosa. ResultsBy RT-PCR examination ,we found that SLP-76 gene over expression in cancer of the digestive tract. ConclusionsDifferent expression of SLP-76 gene in cancer of the digestive tract is found, SLP-76 gene have closely relation with grown in the cancer of digestive tract.It is apossibly potential oncology gene in the cancer of digestive tract. This differential expressio n appeared no tissue specificity with statistical analysis (P=0.9684).更多还原