【摘要】 目的　观察小鼠脾树突状细胞 (DC)与肝癌H2 2 细胞融合瘤苗体内诱导抗肿瘤免疫反应。方法　采用细胞因子诱导、CD11c磁珠标记、MACS分选等技术与传统的聚乙二醇 (PEG)法相结合制备DC与H2 2 细胞融合瘤苗 ,进行致瘤性和诱导CTL活性检测。体内抗肿瘤实验分免疫保护组和免疫治疗组两大组 ,观察融合瘤苗对肿瘤的免疫保护和治疗作用。结果　 1 融合瘤苗接种于小鼠体内未见肿瘤形成 ,脾、肺和肝等器官未出现肿瘤病变。 2 接种活融合瘤苗的免疫小鼠针对H2 2 细胞的脾CTL活性明显高于对照组小鼠 (P <0 0 1)。 3 在免疫保护组 ,经融合瘤苗免疫的小鼠 ,肿瘤潜伏期较对照组明显延长 (P <0 0 5 ) ,肿瘤大小和肿瘤重量均明显小于对照组 (P <0 0 5 )。 4 在免疫治疗组 ,用融合瘤苗治疗的荷瘤小鼠 ,肿瘤大小明显小于对照组 (P <0 0 5 )。结论　 1 DC与H2 2 细胞的融合瘤苗无体内致瘤性 ,已失去其亲本H2 2 细胞的恶性生长特征 ,安全可靠。 2 融合细胞能明显激活小鼠脾特异性的CTL ,提示已获得亲本DC提呈抗原的功能。 3 融合瘤苗对H2 2 细胞的攻击有明显的抵抗作用。 4 融合瘤苗治疗荷瘤小鼠有一定的抑瘤效应 ,而以抵抗肿瘤攻击的免疫保护作用较为显著更多还原

【Abstract】 Aim To develop fusion vaccine of H 22 hepatocarcinoma cell and DC,and study its protective and therapeutic effect against H 22 cell.Methods Fusion vaccine was produced by traditional PEG fusing,cytokine inducing,CD11c magnetic microbead marker sorting and MACS.Pathologically tast the liver,spleen and lung after the fusion vaccine injection.For studying the therapeutical and protective effect of the fusion vaccine against tumor H 22 ,two groups were divided:immune group and therapeutic group.Immune group was divided into P,H,D and HD subgroups,immunized by PBS,inactivated H 22 ,DC and the fusion vaccine respectively,and attacked by H 22 cell.The tumor size,weight,mouse survival period and tumor latent period were recorded and statistically analysized.Therapeutical group was divided into three subgroups of P,D and HD,and attacked by H 22 ,then treated with PBS,DC,and H 22 DC respectively.Pathology and flow cytometry were also applied to study the theraputic effect of the fusion vaccine against H 22 cell.Results 1.No tumor was found in spleen,lung and liver after the fusion vaccine injection;2.The fusion vaccine immunized mice had the strongest CTL activity.3.In immune group,latent period was longer in HD subgroup than others,and tumor size and tumor weight were also smaller in HD subgroup;4.In therapeutic group,tumor size was smaller in HD subgroup than in P,D subgroup.Conclusions 1.The fusion vaccine is have no oncogenesis in vivo;2.The fusion vaccine can stimulate potent specific CTL activity against H 22 ;3.The fusion vaccine has distinctive protective effect against tumor H 22 ;4 The fusion vaccine has certain effect on inhibiting mice tumor,and its immunitive protection against tumor attack is more significance.更多还原