【摘要】 研究了不同pH和乙酸浓度下 ,乙酸对酸性磷酸酶和牛血清白蛋白 (BSA)在土壤胶体、矿物表面吸附的影响。结果表明 ,在pH 2～ 8的乙酸体系中 ,酸性磷酸酶和BSA在胶体矿物表面的最大吸附pH值一般出现在蛋白的等电点和矿物的电荷零点 (PZC)之间。各土壤胶体和粘粒矿物对酶和BSA的吸附量大小顺序为 :针铁矿 黄棕壤 >砖红壤 >高岭石 >二氧化锰。乙酸浓度对蛋白分子在胶体矿物表面的吸附量和吸附结合能具有较显著影响 ,在 0～ 2 0 0mmolL- 1范围 ,随着乙酸浓度的增加 ,蛋白吸附量呈现出先升高 ,后降低 ,再稳定的趋势 ,而吸附结合能的变化与此相反。本文还就乙酸对酶和BSA吸附影响的可能机理进行了初步探讨。更多还原

【Abstract】 Summary Effects of pH and acetate concentration on the adsorption of acid phosphatase and BSA by soil colloid and clay minerals (SCCM) were studied. The results showed that the pH for the maximum adsorption of enzyme and BSA were between the IEP of protein and the PZC of SCCM. In the acetate systems, the amount of enzyme and BSA adsorbed by SCCM increased in the order goethiteyellow brown soil>latosol>kaolinite>δ-MnO 2. Remarkable influences of acetate concentration on the amount of adsorption and the binding energy of enzyme and BSA were observed. With the increase in acetic acid concentration from 0 to 200 mmol L -1 in the system, acetic acid exhibited an initial enhancing effect followed by an inhibiting action on the adsorption of enzyme and BSA on SCCM. The changes in binding energy ( K value) for enz yme and BSA by SCCM were contrary to the trend for maximum adsorption. The possible mechanisms for the influence of acetate on the adsorption of enzyme and BSA by SCCM were discussed.更多还原