节点文献
芦荟多糖的分离纯化及性质研究
ISOLATION,PURIFICATION AND PROPERTY STUDY OF POLYSACCHARIDE FROM ALOE
【摘要】 采用水提醇沉法提取芦荟多糖 ,经DEAE C32柱层析分离 ,SephadexG 10 0进一步纯化 ,得AⅠ、AⅡ和AⅢ三种芦荟多糖。SephadexG 10 0凝胶色谱表明 ,AⅠ组分为均一组分 ,其分子量约为 3 8× 10 4。借助气相色谱技术 ,研究了芦荟粗多糖和AⅠ组分的单糖组成。另外 ,红外光谱鉴定芦荟多糖主要为吡喃多糖
【Abstract】 In this article,polysaccharide in Aloe gel was extracted by water and precipitated by ethanol.It was isolated and purified by chromatography of DEAE cellulose and Sephadex G 100 columns.Three kinds of polysaccharide(AⅠ,AⅡ and AⅢ)were obtained.The results of chromatography of Sephadex G 100 column chromatography showed that AⅠ was uniform.and the molecular weight of AⅠ is 3 8×10 4.The compositions of crude and AⅠ polysaccharide were determined and quantified by GC.In addition,a majority of Aloe polysaccharide is pyranopolysaccharide.
【关键词】 芦荟多糖;
红外光谱;
分离纯化;
气相色谱;
【Key words】 Aloe polysaccharide; IR spectrum; isolation and purification; gas chromatogram;
【Key words】 Aloe polysaccharide; IR spectrum; isolation and purification; gas chromatogram;
- 【文献出处】 天然产物研究与开发 ,Natural Product Research and Development , 编辑部邮箱 ,2003年02期
- 【分类号】O636
- 【被引频次】10
- 【下载频次】508