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芦荟多糖的分离纯化及性质研究

ISOLATION,PURIFICATION AND PROPERTY STUDY OF POLYSACCHARIDE FROM ALOE

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【作者】 王俊玲; 齐祥明; 宗志敏; 魏贤勇;

【Author】 WANG Jun ling 1,QI Xiang ming 2,ZONG Zhi min 1,WEI Xian yong 1 ( 1.School of Chemical Engineering,China University of Mining and Technology ,Xuzhou 221008,China; 2. College of Material and Chemical Engineering,Zhejiang University ,Hangzhou 310027,China)

【机构】 中国矿业大学化学工程学院; 浙江大学材料与化学工程学院; 中国矿业大学化学工程学院 江苏徐州221008; 浙江杭州310027; 江苏徐州221008; 江苏徐州221008;

【摘要】 采用水提醇沉法提取芦荟多糖 ,经DEAE C32柱层析分离 ,SephadexG 10 0进一步纯化 ,得AⅠ、AⅡ和AⅢ三种芦荟多糖。SephadexG 10 0凝胶色谱表明 ,AⅠ组分为均一组分 ,其分子量约为 3 8× 10 4。借助气相色谱技术 ,研究了芦荟粗多糖和AⅠ组分的单糖组成。另外 ,红外光谱鉴定芦荟多糖主要为吡喃多糖

【Abstract】 In this article,polysaccharide in Aloe gel was extracted by water and precipitated by ethanol.It was isolated and purified by chromatography of DEAE cellulose and Sephadex G 100 columns.Three kinds of polysaccharide(AⅠ,AⅡ and AⅢ)were obtained.The results of chromatography of Sephadex G 100 column chromatography showed that AⅠ was uniform.and the molecular weight of AⅠ is 3 8×10 4.The compositions of crude and AⅠ polysaccharide were determined and quantified by GC.In addition,a majority of Aloe polysaccharide is pyranopolysaccharide.

  • 【文献出处】 天然产物研究与开发 ,Natural Product Research and Development , 编辑部邮箱 ,2003年02期
  • 【分类号】O636
  • 【被引频次】10
  • 【下载频次】508
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