【摘要】 目的 :探讨肿瘤坏死因子 (TNF -α)注入兔左肾动脉壁内引起动脉炎后 ,左肾脏形态学改变及其机理的研究。方法:用光镜、免疫组化、电镜观察肾脏的变化。结果:肾小球系膜细胞轻度增生 ,毛细血管基底膜增厚 ,系膜基质增多。肾小球基底膜及肾小管基底膜增厚。增殖细胞核抗原 (PCNA)于左肾脏髓质集合管上皮细胞核呈阳性反应。电镜 :肾小球的内皮细胞、足细胞、肾小球系膜细胞及系膜区基质均处于增生状态。肾髓质成纤维样细胞和BohmanⅠ型细胞轻度增生 ,近曲小管上皮胞浆空泡变。结论:少量TNF -α可以引起肾脏增生样改变 ,TNF -α的拮抗剂可能对肾脏有保护和治疗的作用更多还原

【Abstract】 Objective:To observe the secondary renal pathological features induced arteritis in rabbits by injected TNF-αinto renal arterial walls.Methods:With light microscope?immunoˉhistochemistry and electronic microscopy to observe the renal pathological injuries.Results:The renal morphological changes include glomerular lesions:mesangial cells hyperplasia and increased mesangial matrix.The GBM and TBM become thickening.Immunohistochemistry stain shows PCNA positive in epidermal cells of collecting tubes.Electronic microscopy examiˉnation shows Bohman-Ⅰcells’s hyperplasia in medulla.Conclusion:A few amount tumor necrosis factor(TNF-α)can cause renal hyperplasia injuries.更多还原