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转基因黄瓜主要农艺性状观察及其种子抗性研究

Studies on Main Agricul tural Trait of Transgenic Cucumber and Its Seed Resistance

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【作者】 金红杜胜利陈峥张力魏爱民

【Author】 JIN Hong 1 ,DU Sheng-li 2 ,CHEN Zheng 1 ,ZHANG Li 2 ,WEI Ai-min 2 (1.Central Lab of Tianjin Academy of Agricultural Sciences,Tianjin300192,China;2.Tianjin Kerun Cucumber Institute,Tianjin300192,China)

【机构】 天津市农业科学院中心实验室天津市科润黄瓜研究所天津市科润黄瓜研究所 天津300192天津300192天津300192

【摘要】 通过对转基因黄瓜和非转基因黄瓜田间农艺性状的观察,转基因黄瓜的T1代植株与对照亲本在主要农艺性状上没有差异。不同转基因株系自交后的T1代种子对除草剂BASTA的抗性各不相同,但远远高于对照。经过除草剂处理后的抗性种芽的叶绿素含量显著高于非抗性种芽,而非抗性种芽的可溶性蛋白总量和可溶性糖总量则因受除草剂的毒害生长缓慢而高于抗性芽,这也反证了转基因抗除草剂种子的生长。

【Abstract】 By watching the field agricultural traits of transgenic and non-transgenic cucumber plants,no differences were found between T1transgenic plants and ck.Self-crossing T1seeds of different T1transgenic lines showed different resistances to BASTA,but their resistances were all higher than those of non-transgenic seeds.After treated by herbicide,the chlorophyⅡcontents of resistant shootings were significantly higher than those of non-resistant shootings;due to toxic effect of herbicide,non-resistant shootings grew more slowly,thus their total contents of soluble protein and soluble carbohydrate were higher than those of resistant shootings,this result just proved the growth of transgenic seed.

【基金】 天津市自然科学基金项目(003802611)
  • 【文献出处】 天津农业科学 ,Tianjin Agricultural Sciences , 编辑部邮箱 ,2003年01期
  • 【分类号】S642.2
  • 【被引频次】2
  • 【下载频次】149
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