【摘要】 核苷酸和 β_环糊精 (β_CD)及硼酸盐之间存在络合作用 ,可应用于核苷酸的高效毛细管电泳分离 ,在10mmol/Lβ_CD和20mmol/L硼酸盐存在时 ,实现了8种单磷酸核苷酸的分离测定 ;讨论了 pH值、β_CD浓度、硼酸盐浓度及分离电压对分离和络合作用的影响更多还原

【Abstract】 The electrophoretic behavior of nucleotides can be manipulated using complex _ forming reactions with β _ cyclodextrin(β _ CD) and borate. A mixture of 8 monophosphate nucleotides can be separated by high performance capillary electrophoresis using a buffer containing 20 mmol/L borate-10 mmol/L β _ CD. The effect of pH, con _ centrations of β _ CD and borate, and separation voltage on the separation and complexation are discussed.更多还原