【摘要】 以腐蹄病A型节瘤拟杆菌染色体DNA为模板,根据设计的上、下游引物进行PCR反应,扩增出了大小约0.78kb的基因片段,用T₄DNA连接酶将PCR产物与pGEM-T-Easy载体连接,构建了重组质粒。并以EcoRV和SalI双酶切重组质粒鉴定目的基因的插入方向。经序列测定,克隆的目的基因片段为Pili基因。更多还原

【Abstract】 The pili gene that dominates the main protective antigen was amplified and cloned from D. nodosus serotype A by PCR. A recombinant plasmid,designated TE- Pili,was constructed by inserting the Pili gene intoT - Easy which was digested with EcoRV and Sal I. The result of sequencing showed that the fragment was exactly Pili gene of D. nodosus serotype A.更多还原