【摘要】 目的　检测大肠癌患者外周血中组织特异性 m RNA的表达并分析其临床意义。方法　用 RT- PCR方法检测 70例大肠癌患者和 35例正常健康人外周血中组织特异性 m RNA的表达。结果　 70例大肠癌患者中有 30例外周血中组织特异性 m RNA表达阳性 ,阳性率为 42 .9% ,与健康对照人群相比差异有显著性 ( P<0 .0 5 ) ;DukesC和 Dukes D期大肠癌患者外周血中组织特异性 m RNA的表达高于 Dukes A期大肠癌患者 ( P<0 .0 5 ) ,在不同分化程度大肠癌患者外周血中的表达差异无显著性 ( P>0 .0 5 ) ,手术后比手术前的阳性表达率低 ,但差异无显著性( P>0 .0 5 )。结论　大肠癌患者外周血中组织特异性 m RNA的阳性表达同肿瘤的分期有关 ,可作为大肠癌微转移的一个监测指标。更多还原

【Abstract】 Objective To investigate the histospecific mRNA expression in peripheral blood cells from patients with colorectal cancer and to evaluate its clinical significance. Methods Peripheral blood samples were collected from 70 eligible patients with colorectal cancer. Histospecific mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Results The positive rate of histospecific mRNA of 70 patients with colorectal cancer was 42.9% and there was a significant difference compared with healthy volunteers. The positive rate increased with the pathological stages significantly and showed no significant relationship with cell differentiation. Conclusion The expression of histospecific mRNA in peripheral blood cells correlates with the pathological stages of colorectal cancer.;更多还原