【摘要】 目的　探讨缺氧缺血再灌注后海马神经元的存活机制。方法　 5 6只 7日龄SD鼠随机分为假手术对照组、缺氧缺血脑损伤 (HIBD)组。制备HIBD并再灌注模型。多普勒监测血流变化。免疫组化检测反应元件结合蛋白磷酸化 (p CREB)在HIBD不同再灌注时期海马区的表达。Thionin染色及Turnel法检测神经元的凋亡。结果　HIBD再灌注 3、2 4h仔鼠右侧海马各区p CREB表达达高峰 ,7d后降至对照组水平 ;对照组两侧 p CREB有基础表达 ,但无差别。检测凋亡发现 :右侧海马锥体细胞在HIBD再灌注 2 4h后已有明显凋亡 (P <0 .0 1) ,但 7d后神经元无明显丢失。假手术组海马极少数神经元凋亡。结论　p CREB可能是神经元HIBD后修复、存活的关键因素更多还原

【Abstract】 Objective To explore the survival mechanism of hippocampal neurons after the damage of hypoxic ischemia and reperfusion of the brain.Methods Seven days old SD rats (n=56) were randomly divided into HIBD group and sham group. The HIBD and reperfusion model was established. The flow of blood was detected by the multicolor Doppler.The p cAMP response element binding protein p CREB was immunohistochemically evaluated at different time point of reperfusion in the region of rat′s hippocampus.Results The expression of p CREB reached the peak at 3 h and 24 h post reperfusion in the right hippocampus of HIBD group, and then decreased to the normal level on the seventh day.In control group the same regions showed basic immunoreactivity with the specific antibodies. Thionin staining and Turnel detecting revealed that a partial pyramidal neurons of right hippocampus showed the character of apoptosis at 24 h post reperfusion. But on the 7 d of recirculation the loss of neurons had no marked distinction (P>0.05). The sham animal showed very few apoptosis cells in the regions of hippocampus.Conclusion During the period of post hypoxic ischemia reperfusion the phosphorylation of CREB may be a key factor for the survival and repairment of hippocampal neurons on the ischemic side.更多还原