【摘要】 目的 探讨缺氧缺血性脑损伤(HIBD)后学习记忆行为功能与突触可塑性之间的关系。方法 7日龄Wistar大鼠36只随机分为缺氧缺血(HI)组21只和正常对照组15只。HI组大鼠通过结扎左侧颈总动脉后吸入8％的氮氧混合气体2h,制成缺氧缺血性脑病(HIE)模型。饲养至80d左右时,采用Y-迷宫实验检测分辨学习和记忆能力,免疫组织化学方法对海马结构突触素的表达进行分析。结果 HI组大鼠分辨学习能力和记忆保持百分数分别为33±8和(59±21)％,显著低于正常对照组27±7和(80±19)％(P<0.01,P<0.05);海马、齿状回突触素免疫染色出现明显的免疫板层分布,HIE组海马CAI区始层、辐射层、腔隙分子层及齿状回分子层突触素免疫产物校正光密度(COD)值均较对照组明显降低(P<0.01),分别降至41.2％,27.2％,10.5％和38.9％。结论 HIBD后大鼠学习和记忆能力明显下降,可能与海马结构突触素减少有密切关系。更多还原

【Abstract】 Objective To study the relation of learning and memory abilities and expression of hippocampal synaptophysine after hypoxic-ischemic brain damage in neonatal rats. Methods Thirty six seven-day-old postnatal rats were divided into the hypoxic-is-chemic(HI) group ( n = 21) and the control group ( n = 15) randomly. The rats of HI group were subjected to left common carotid artery ligation followed by exposure to 8 % oxygen at 37 ℃ for 2 h. When the rats were 80 to 90-day-old, the retaining percentage of their discriminative learning and memory were measured by test of Y-maze. The expression of synaptophysins was estimated immuno-histochernically in paraffin sections of hippocampal formation. Results The Y-maze discriminative learning abilities (33 ± 8) and memory-keeping percentage (59 ± 21) % in the HI rats were decreased significantly than those of the control [27 ± 7 and (80 ±19) % , P < 0.01,P < 0.05 ]. The laminar distribution patterns of immunoreactive products of anti-synaptophysin were found in the hippocampal formation. The corrected optical densities (COD) of immunoreactive products of the hippocampus and dentate gyrus were significantly decreased in HI rats in comparison with normal rats, and decline to 41.2 %,27.2 %,10.5 % and 38.9 % respectively. Conclusion Hypoxic-ischemic brain damage may lead rats to disorders of learning and memory abilities, which may be associated with the reduction of synaptophysine in hippocampal formation.更多还原