【摘要】 目的 :观察胰岛素对体外培养的人牙周膜 (periodontalligament ,PDL)细胞DNA合成和细胞周期的影响 ,探讨胰岛素促进PDL细胞增殖的可能机制。方法 :采用体外细胞培养技术、3H TdR掺入法和流式细胞术 ,检测胰岛素对人PDL细胞DNA合成和细胞周期的影响。结果 :胰岛素浓度在 1～ 10 0 0U /L之间时明显增加人牙周膜细胞的3H TdR掺入率 ,当浓度大于 10U/L时效应维持在稳定状态。在胰岛素作用下 ,细胞G1%较对照组降低 ,而S %和G2 M %增高。结论 :胰岛素能促进人PDL细胞的DNA合成 ,刺激细胞由G1期向S期转化更多还原

【Abstract】 Objective: To investigate the effects of insulin on the DNA synthesis and cell cycle distribution of human periodontal ligament cells (PDLs). Methods:PDLs were treated with insulin at the doses (U/L) of 0.01～1 000 for 72 h,DNA synthesis of the cells was measured by 3H-TdR incorporation and cell cycle distribution was studied by flow cytometer. Results : Insulin at 1 U/L to 1 000 U/L showed a significant concentration dependent stimulation of 3H thymidine uptake, and the effect of insulin at 100 ～ 1 000 U/L were not statistically different from that at 10 U/L. The percentage of PDL cells in G1 phase during exponential growth was decreased by insulin, while that in S phase and (G2+M) was increased in experimental group. Conclusion: Insulin increases DNA synthesis of PDL cells ,and stimulates the transformation of cells from G1 to S phase.更多还原