【摘要】 目的 :评价碱性成纤维细胞生长因子 (bFGF)对成骨的促进作用 ,以期为颌骨缺损提供一种较为理想的替代材料。方法 :18只成年兔双侧下颌骨下缘各造成 15mm× 6mm的全层骨膜骨质缺损 ,每一缺损作为一个实验单位 ,按自身同期配对设计 ,分别植入复合骨和天然型无机骨 (NNB)。术后 3、6、12周取下标本 ,进行甲苯胺蓝染色观察、四环素荧光检查以及组织学成骨面积定量分析。结果 :复合骨新骨形成及钙化早 ,同期成骨面积大于NNB(P <0 .0 1)。结论 :合适的复合条件下bFGF可有效地促进软骨及骨的生长 ,复合骨新骨形成及钙化早 ,同期成骨量多 ,修复效果优于NNB。更多还原

【Abstract】 Objective: To study the effects of basic fibroblast growth factor(bFGF) on promoting new bone formation in repairing mandibular defects.Methods: 15 mm×6 mm bilateral mandibular periosteum bone defects were made surgically in 18 adult New Zealand rabbits, the right defects were repaired with natural non organic bone (NNB) combined with bFGF at 40 ng/mm 3, the left defects were repaired with NNB. Specimens were obtained 3, 6, 12 weeks after operation respectively. The effects were evaluated with toluidine blue stain observation, tetracycline fluorescent microscopic examination and computerized quantity analysis. Results: More chondrocytes were observed in the right defects 3 weeks after operation. 3, 6, 12 weeks after operation the new bone formation (μm 2) in the right defects was 66.092±6.379, 130.198±13.213 and 235.374±16.773 respectively; that in the left defects 21.844± 7.731 , 62.694±10.389 and 160.184±14.793 respectively( P< 0.01). Conclusion: Under the proper combination, bFGF can effectively increase chondrocyte proliferation and new bone formation.更多还原