【摘要】 目的　建立检测血清风疹 Ig G ( RV - Ig G)抗体的间接酶联免疫测定 ( enzymelinkedim munosorbent assay,EL ISA)方法。　方法　采用 RV - GOS株感染单层 Vero细胞方法制备 RV抗原 ,并采用血凝素方法提纯抗原 ,建立定量间接 EL ISA方法 ,进行了实验条件的选择。然后用优化的 EL ISA法对检测 RV- Ig G的敏感性、特异性和重复性进行鉴定 ,并与美国食品与药品监督局认证的 EL ISA方法进行比较。　结果　间接定量 EL ISA方法的敏感度为 3IU / m l,以 Diasorin公司生产的 RV - Ig G试剂盒检测结果为标准 ,其敏感性、特异性和准确性分别为 97.79% ( 2 2 1/ 2 2 6 )、95 .4 5 %( 4 2 / 4 4 )、 97.4 1% ( 2 6 3/ 2 70 ) ;2种方法相关系数为 0 .92 ,P<0 .0 5 ,呈显著相关 ;　结论　该 EL ISA方法敏感性高、特异性强 ,且简便、快速 ,重复性好 ,具有实际应用价值更多还原

【Abstract】 Objective To establish an indirect Enzyme linked immunosorbent assay(ELISA)method for detecting rubella IgG antiboby in serum. Methods The rubella antigen was extracted from succession of culture monolayer Vero cell infected with RV GOS strain.An indirect ELISA was developed.The sensitivity,specificity,repetition were evaluated and compared with the ELISA approach provided by Diasorin Co. Results The sensitivity of the indirect ELISA quantitative assay is 3 IU/ml.The indirect ELISA assay yielded 221/226 positive results,resulting in a sensitivity of 97 79 %.The specificity was 95 45 %(42/44),and accuracy was 97 41 %(263/270).The relative coefficient (r) between two methods was 0 92 (P<0 05). Conclusion The indirect ELISA method is specific and sensitive,reliable and simple,and can be used for screening the rubella antiboby in premarital women.更多还原