【摘要】 为了克隆福氏志贺氏菌的ipaC基因,以志贺氏菌福氏2a菌株为模板进行PCR,并将PCR产物插入到pMD T载体中,得到阳性重组子,并命名为pMD ipaC.测序结果显示,其核苷酸序列与文献报道的序列有差异,但编码的氨基酸序列相同,表明志贺氏菌的ipaC基因已被成功克隆.更多还原

【Abstract】 To clone ipaC gene of Shigella flexneri , PCR is performed with the stains of S. flexneri 2a as template, and the PCR product is inserted into pMD-T vector. The positive recombinant is obtained and designated as pMD-ipaC. Sequencing results show that there are differences in nucleotide sequence with the reported, but the coded amino sequence is the same, indicating ipaC gene cloned successfully. This provides the basis for current understanding of the function of IpaC in the pathogenesis of S. flexneri further.更多还原