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小麦高分子量谷蛋白亚基近等基因系的研究
Studies on HMW-GS near-iso genic Lines of Wheat
【摘要】 以安农8455为母本,Pan555为父本组合杂交,并采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法(SDS PAGE)对安农8455/Pan555的620粒F2籽粒的高分子量麦谷蛋白亚基(HMW GS)进行了测定,结果表明:F2籽粒中共出现了15种带型组合,可稳定出多对近等基因系;Glu A1a与Glu A1c分离比为1∶1;Glu B1b与Glu B1c分离比为9∶7;Glu D1d与Glu D1i分离比为1∶1;Glu B1b与或Glu B1c的不完全表达率为4 0%。
【Abstract】 Annong8455 were crossed with Pan555,and F2 seeds were tested by 10% SDS\|PAGE for the HMW\|GS.The results showed that 15 different band patterns were occurred,the ratio of \%Glu\|A1a\% to \%Glu\|A1c\% was about 1 to 1, the ratio of \%Glu\|B1b\% to \%Glu\|B1c\% was about 9 to 7 , the ratio of \%Glu\|D1d\% to \%Glu\|D1i\% was about 1 to 1,the incomplete expression rate of \%Glu\|B1b\% and/or \%Glu\|B1c\% was 40%.
【关键词】 小麦;
十二烷基硫酸钠聚丙烯酰胺凝胶电泳法;
高分子量麦谷蛋白亚基;
近等基因系;
【Key words】 Wheat; SDS\|PAGE; HMW\|GS; Near-isogenic lines;
【Key words】 Wheat; SDS\|PAGE; HMW\|GS; Near-isogenic lines;
【基金】 国家948项目(201006C);山西省自然基金资助项目(2002182)
- 【文献出处】 山西农业大学学报(自然科学版) ,Journal of Shanxi Agricultural University , 编辑部邮箱 ,2003年03期
- 【分类号】S512.1
- 【被引频次】7
- 【下载频次】96