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香菇半亲和双单杂交研究初报
A preliminary study of hemi-compatible di-mon mating in lentinula edodes
【摘要】 选用一栽培株苏香两种孢子单核体自交后代作双核体亲本 ,与相应的孢子单核体进行半亲和双单杂交 ,得到 6个杂交后代 ,均具有结实能力 ,说明半亲和双单杂交后代可以用于育种研究。大多数杂交菌株 (核基因相同 )在菌丝生长速率与CMC酶活方面显示出不同程度遗传差异 ,其中 3号与 4号菌株在菌丝生长速率与CMC酶活方面呈现明显杂种优势。
【Abstract】 The selfed progenies of sporualated monokaryons of a cultured strain in Lentinula edodes and their responding sporualated monokaryons were selected for hemi-compatiable di-mon mating.Six hybrids were obtained from their culture,which generated primordia in a liquid medium. Most hybrids of the same genome indicated the different genetic differences in the growth rate of mycelium and CMC,and No.4 of which showed obvious hybrid vigour.
【关键词】 香菇;
半亲和双单杂交;
液体出菇;
菌丝生长速率;
CMC酶活;
杂种优势;
【Key words】 Lentinula edodes; hemi-compatiable di-mon mating; primordia formation in a liquid medium; growth rate of mycelium; CMC enzyme activity; hybrid vigour;
【Key words】 Lentinula edodes; hemi-compatiable di-mon mating; primordia formation in a liquid medium; growth rate of mycelium; CMC enzyme activity; hybrid vigour;
【基金】 安徽省教育厅科研基金 (2 0 0 0j1 0 2 9);安徽省自然科学基金 (1 0 1 4 1 1 1 2 )资助
- 【文献出处】 生物学杂志 ,Journal of Biology , 编辑部邮箱 ,2003年01期
- 【分类号】S646
- 【被引频次】3
- 【下载频次】107