【摘要】 小麦种子水培 ,幼苗长至一叶一心后 ,在 2 0℃生长箱中水培 4 8h作为对照组 (Driver) ,PEG 6 0 0 0水溶液胁迫培养 4 8h作为处理组 (Tester)。进行抑制差减杂交 ,构建包含 15 0 0个独立克隆的SSH文库。以正向和反向差减杂交后的cDNA为探针 ,筛选SSH文库 ,得到 181个阳性克隆 ,测序后获不重复EST 10 1个。更多还原

【Abstract】 Hanxuan No.10 wheat (Triticum aestivum L.) seedlings were cultivated in water at 20℃ with 12h light every day until one and a half leaves. Then half seedlings were continuously cultivated in water for 48h as control (the driver), another half seedlings were stressed in PEG 6000(-0.5MPa)solution for 48h as treatment (the tester). A subtracted cDNA library was constructed by suppression subtractive hybridization (SSH), which was composed of approximately 1500 individual recombinant clones. A total of 181 positive clones were obtained by screening the library using forward and reverse subtracted cDNA probes, and 101 unique ESTs induced by water stress were gained based on the sequencing data.更多还原